Journal: Neoplasia (New York, N.Y.)

Article Title: SAMSN1 Is a Tumor Suppressor Gene in Multiple Myeloma 1 2

doi: 10.1016/j.neo.2014.07.002

Figure Lengend Snippet: SAMSN1 expression is reduced in CD138 + PCs of patients with MM and HMCL. (A) SAMSN1 expression (as determined by real-time PCR) is significantly reduced in the BMs of patients with MM ( n = 34) compared with patients with MGUS ( n = 9) and healthy age-matched controls ( n = 5; * P < .05, ** P < .001, one-way ANOVA with Tukey’s multiple comparison test). (B) SAMSN1 expression in CD138 + MACS isolated PCs from patients with MM negatively correlates with BM PC burden ( n = 10, r 2 = 0.6147, P = .0043). (C) In silico analysis of published microarray data. CD138 + PCs were isolated by MACS from 414 patients with MM, 44 patients with MGUS, and 22 age-matched controls. RNA was extracted and analyzed using the Affymetrix U133Plus2.0 microarray platform (GEO Accession Nos GSE4581 and GSE5900). Expression of SAMSN1 is significantly reduced in PCs of patients with MM compared to those of patients with MGUS and normal controls. P < 0.0001, one-way ANOVA with Tukey’s multiple comparison test. (D) Total RNA was extracted from six HMCLs and reverse transcribed. The levels of SAMSN1 expression were assessed by real-time PCR.

Article Snippet: PCs were isolated from flushed long bones and identified using rat anti-mouse CD138 (R&D Systems, Minneapolis, MN) followed by PE-conjugated goat anti-rat IgG.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Comparison, Isolation, In Silico, Microarray, Reverse Transcription

Journal: Biology of reproduction

Article Title: Interleukin 4 and interleukin 13 downregulate the lipopolysaccharide-mediated inflammatory response by human gestation-associated tissues.

doi: 10.1095/biolreprod.116.145680

Figure Lengend Snippet: Figure 3. Anti-inflammatory effects of IL4 and IL13 in the placenta, choriodecidua, and amnion are IL4RA dependent. Cytokine levels (IL1B and MIP1A) from explants of (A, B) placenta, (C, D) choriodecidua, and (E, F) amnion in response to LPS (10 ng/ml) pretreated for 90 min with IL4 or IL13 (both 10 ng/ml) in the presence or absence of mouse IgG2a anti-human IL4RA antibody or mouse IgG2a isotype. Data are shown as % of LPS alone (n = 3); error bars represent SEM. Statistically significant differences compared to LPS + IL4/13 treatments are shown: ∗P ≤0.05, ∗∗P ≤0.01.

Article Snippet: All cultures were incubated for 24 h. For inhibition/neutralization experiments, cultures were incubated with the following antibodies: anti-hIL4RA IgG2a, and Mouse IgG2a isotype (both 1 μg/ml; R&D Systems, USA) for 30 min prior to the addition of cytokines/LPS.

Techniques: